bioinformatics
.tools
Tools
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Calculators for sequence analysis, cloning and assay data
Each tool is a single page that runs in the browser. No account, no upload.
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Sequence
Reverse complement
DNA to RNA
Translate DNA to protein
Six-frame translation
ORF finder
Reverse translate
Split codons
Sequence statistics
Sequence length
DNA and RNA length
GC and AT content
GC skew
k-mer counter
Pattern find
Fuzzy search
Tandem repeat finder
CpG islands
IUPAC expander
RNA secondary structure
Codons and expression
Codon usage
Codon optimizer
Rare codon analysis
CpG depletion
AAV component explorer
mRNA extinction coefficient
Primers and PCR
Melting temperature
Melting curve plotter
Primer designer
Primer map
PCR product size
PCR master mix
PCR extension time and annealing
Oligo calculator
Primer dimer and hairpin
CRISPR guide finder
Cloning
Restriction sites, digest and map
Mutate for digest
Ligation calculator
Gibson assembly
Concatenate genes
Transformation efficiency
Quantitation and dilution
Nucleic acid mass to moles
DNA copy number
Molarity
Dilution
Serial dilution
Antibody dilution
Beer–Lambert
Percent solution
Buffers and units
Henderson–Hasselbalch
Phosphate buffer
g-force to rpm
Temperature converter
ΔG to Kd
Agarose gel percentage
Buffer recipes
Michaelis–Menten
Protein
Protein mass to moles
A280 concentration
Protein concentration assay
Protein statistics
Amino acid code converter
Alignment
Needleman–Wunsch
Smith–Waterman
Pairwise protein
Pairwise codons
BLAST-style alignment
DNA compare
Identity and similarity
Alignment quality scorer
Alignment trimmer
Merge alignments
Color by conservation
Phylogenetic tree (UPGMA)
Scoring and statistics
Scoring matrix
BLAST E-value
Shannon entropy
Culture and assay
OD600 to cell count
OD growth
Bacterial growth
Doubling time
Colony forming units
Cell viability
ELISA analyzer
Z-factor
Hemocytometer cell count
MOI calculator
TCID50
Dose–response
IC50
EC50
LD50
LC50
Ki from IC50
Dose–response curve fitter
Synergy
Random and shuffled sequences
Random DNA
Random DNA regions
Sample DNA
Shuffle DNA
Mutate DNA
Cleanup and formatting
FASTA cleaner
Gap cleaner
Case converter
Sequence formatter
Sequence interleaver
Range extractor DNA
Window extractor DNA
Combine FASTA
FASTA deduplicator
List compare
File conversion
GenBank to FASTA
FASTQ to FASTA
FASTA to FASTQ
Alignment format converter
qPCR and gene expression
ΔΔCt relative expression
qPCR efficiency
Reference
Codon table
Amino acid table